RESUMO
Background: in this study, we compared the effect of ibuprofen [IB] while incorporating by Poly Lactic-co-Glycolic Acid [PLGA] nanofiber on expression of adhesion molecules ICAM-1 and VCAM-1 in a mice adhesion model
Materials and Methods: using an adhesion model were induced in mice, PLGA-IB and PLGA membranes and IB were sutured between the abdominal wall and peritoneum after surgical operation to reveal the best membrane for prevention of postoperative adhesion bands by comparison of ICAM-1 and VCAM-1 expression
Results: compared with other groups, PLGA-IB showed a greater ability to reduce ICAM-1 and VCAM-1 expression
Conclusion: these results suggested that in considering the FDA approved polymers, PLGA-IB could be introduced as a potential candidate for prevention of abdominal post-surgery inflammation and adhesion band formation after surgeries
RESUMO
Objective: this study examined the in vivo differentiation of mesenchymal stem cells [MSCs] into insulin producing cells [IPCs] on electrospun poly-L-lactide acid [PLLA] scaffolds coated with Matricaria chammomila L. [chamomile] oil
Materials and Methods: in this interventional, experimental study adipose MSCs [AMSCs] were isolated from 12 adult male New Zealand white rabbits and characterized by flow cytometry. AMSCs were subsequently differentiated into osteogenic and adipogenic lines. Cells were seeded onto either a PLLA scaffold [control] or PLLA scaffold coated with chamomile oil [experimental]. A total of 24 scaffolds were inserted into the pancreatic area of each rabbit and placement was confirmed by ultrasound. After 21 days, immunohistochemistry analysis of insulin-producing like cells on protein levels confirmed insulin expression of insulin producing cells [IPSCs].Real-time polymerase chain reaction [PCR] determined the expressions of genes related to pancreatic endocrine development and function
Results: fourier transform infrared spectroscopy [FTIR] results confirmed the existence of oil on the surface of the PLLA scaffold. The results showed a new peak at 2854 cm[-1] for the aliphatic CH[2] bond. Pdx1 expression was 0.051 +/- 0.007 in the experimental group and 0.009 +/- 0.002 in the control group. There was significantly increased insulin expression in the scaffold coated with chamomile oil [0.09 +/- 0.001] compared to control group [0.063 +/- 0.009, P
Conclusion: the pancreatic region is an optimal site for differentiation of AMSCs to IPCs. Chamomile oil [as an antioxidant agent] can affect cell adhesion to the scaffold and increase cell differentiation. In addition, the oil may lead to increased blood glucose uptake in pathways in the muscles, liver and fatty tissue of a diabetic animal model by some probable molecular mechanisms
RESUMO
Micropatterning is becoming a powerful tool for studying cells in vitro. This method not only uses very small amount of material but also mimic the microenvironment structure present in living tissues better than flash cul-turing techniques. In previous studies using micropatterning of extracellular matrix proteins on glass surfaces, the rate of protein detachment from the surface was so high that the proteins and the cultivated cells detached after 3 three days of cell seeding. Here we optimized the glass surface modification method to fulfill the requirement of most in vitro studies. in our study we showed that the optimum time for glass surface modification reaction is 1.5 hr, and the cells could be tracked in vitro for over 15 days after cell seeding which is enough for the most in vitro studies. As a model, we cultivated HEK 293T and HepG2 cells on the collagen micro-patterns and showed that they have normal growth and morphology on these micropatterns. The HEK cells also transfected with pmaxCFP plasmid vector to show that the cells on collagen micropatterns could also used in trans-fection studies. Taking these together, this novel method is promising for efficient cell culture studies on micropatterened surfaces in the future